The propagation of aloe vera generally adopts the ramets method or the bud interpolation method, which has a low propagation coefficient and is limited by the season. The use of tissue culture to induce callus and tufts, followed by the expansion of the subculture, in the short term can obtain a large number of provenances, and can be produced all year round, especially in the introduction of new varieties, it can show its superiority. First, materials and methods 1. Name of plant: Aloe 2, material categories: young stem segments 3, culture conditions: MS medium as the basic medium, additional: 1BA (cytokinin) 0.5-3 mg / liter (the same unit below) ten NAA (naphthalene acetic acid) 0.01-0.5; 2BA0.5-1 ten NAA0 .01 10 GA3 (gibberellin) 0.2-1; 2 IBA (indole acetic acid) 0.2-0.4 10 CCC (Cecholine) 1; culture temperature 23-27 °C; light 1500-2000 lux, light 10 hours per day. 4. Experimental method: take stem segments of growing young plants, after disinfection, separate the explants on the ultra-clean bench to 0.5-1 cm, inoculate in medium 1, and conduct induction culture. After 2-3 weeks, Explants begin to form a large number of callus and a small number of clustered shoots; then transferred to medium 2, a large number of clustered shoots begin to form after one week; when the shoot height is 1.5-2 cm, they are separated and transferred to medium 2 In three weeks, a complete plant can be formed. The seedling height is 3-5 cm and the root length is about 2 cm. For mass reproduction, callus can be subcultured in medium 1 for expansion. 2. Establishment and storage of clones Callus of aloe can be stored as a provenance at 5°C. After 5 months of storage in a refrigerator at about 5°C, normal callus induction can still be induced. The stored callus should be cultured in the culture room for three days first to observe whether there is contamination, and then expand the culture. After the experiment, basically no staining is observed, and the refreshing medium can be stored for a long time. Third, the transplanting and management of the test tube seedlings The adaptability of the test tube seedlings to the external environment is poor and must be cultivated after being trained. The method is to open the cork before the seedlings are removed, and still keep the seedlings in the cultivation room for 2-3 days. When taking the seedlings, the roots should be avoided and the medium should be washed in the room temperature water to prevent rottenness. root. Substrate can be used to add appropriate amount of vermiculite or all vermiculite in garden soil. After it is planted, it is topped with a nutrient solution, covered with a thin film, moistened with heat, moisturized for 30 minutes every day, and transplanted and planted four weeks later. RP International Ltd is top Food Acidulants Supplier and Manufacturer in China. Food Acidulants Food Acidulants,Magnesium Citrate,Calcium Citrate Powder,Potassium Citrate RP International Ltd , http://www.rp-foodadditives.com
Acidulants are additives that give a sharp taste to foods. They also assist in the setting of gels and to act as preservatives. The pH of a food is a measure of its acidity, alkalinity or neutrality. Living tissues contain solutions called buffers which help to keep a constant pH inside cells.
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