MF-10 Gradiflow System - A New Approach to Primary Plasma Separation in Proteomics Analysis

Low-abundance proteins, although rare, often carry important information. In the treatment of diseases, they often serve as markers for diagnosis, prognosis, and therapeutic effects, and may also be targets for treatment. In proteomic analysis, researchers also hope to obtain low-abundance proteins in complex samples and find members with special effects. However, like a needle in a haystack, they are not easy to find. In recent years, researchers are constantly proposing new methods and techniques to pick out low-abundance proteins from biological samples to understand exactly what they are and what they mean.

The low-abundance protein of plasma has become a hotspot in scientific research. More and more evidences show that many low-abundance proteins in plasma are important disease markers, and their abnormal expression is closely related to the development of the disease. 2-DE (two-dimensional electrophoresis) is an effective method for separating proteins of different molecular weights and PI values, but because a large number of low-abundance proteins are often combined with high-abundance proteins, the two-dimensional electrophoresis of large-volume complex samples is not ideal. .

Many researchers are doing their best to find more ways to get these low-abundance proteins. But imagine that these low-abundance proteins are bound or masked by high-abundance proteins, and the difficulty of the work can be imagined. Australia MinomicAnna Fitzgerald decided to use the MF-10 Gradiflow system (now known as ProteomeSep, produced by the Australian NuSep company), plasma proteins separated by preliminary separation step, the sample was then by 2-DE separation, hope get better The result of the separation.

The MF-10 Gradiflow system allows for the rapid and easy separation of proteins from complex samples by different protein molecular weights and PI values. Since the sample does not require denaturation treatment when it is separated , the MF-10 separated sample retains the original activity.

Separated by molecular weight. Both sides of the separation column is NMWCO 5kDa membrane of two, placed in the middle of 1000, 500, 150, 65 and 45 kDa   The NMWCO membrane divides the column into six chambers. At the cathode point sample, a certain voltage is applied, and proteins of different size ranges will be separated in different chambers.

Isolation by protein PI value. The 5 1000kDa NMWCO membrane placed in the middle of the column, the column is divided into six slots chamber. Samples are taken from each chamber, voltage is applied, and the samples are separated according to the PI value.

Anna Fitzgerald used MF-10 to separate plasma samples for better results. What the researchers did was a simple two-step electrophoresis. However, the specific gravity of the high-abundance protein in the sample is greatly reduced, so that the separation difficulty is greatly reduced.

The MF-10 Gradiflow system solves the problem that the low-abundance protein is difficult to separate in complex samples. It is convenient and quick to operate. It can be used not only in plasma proteomics research, but also in the analysis of cerebrospinal fluid and urine. Applications.

MF-10 Gradiflow System Product Introduction

Hong Kong Bo Qi Technology Co., Ltd.
Guangzhou Boqi Biological Technology Co., Ltd.
400 600 9639


Hong Kong Beijing Shanghai Guangzhou Xiamen Kunming