Pipette pipetting technique for laboratory instruments 7 – Reverse pipetting mode

I believe that every laboratory worker has experience in using pipettes, and also requires high accuracy and accuracy of the pipette, but in addition to the accuracy and accuracy of the pipette itself will affect our shift. In addition to the liquid results, The use of pipettes also has an impact on our pipetting results. In order to get the best results and ensure the accuracy of the experiment, we need Use good pipetting techniques. Lebe will introduce you to tips and tricks for getting the best pipetting results.

Pipetting Tip: Reverse Pipetting Mode
For our researchers, the use of pipettes for precise aspiration and dispensing of viscous and dense liquids has been a challenge. The best countermeasure is to use a pipette with reverse pipetting mode (such as an IKA pipette ). The specific operations are as follows:

When we are doing sample aspiration, we can press the locating pin down to “ drain ” to draw the selected liquid and excess liquid into the pipette tip. Then, when dispensing, press the positioning pin only to the zero position. By this method, the liquid remains in the tip during dispensing. For biological, viscous or foaming liquids, reverse pipetting can improve the results. However, if the results need absolute precision, then   The IKA Precision range of pipettes can improve these results even more dramatically.

on   IKA   For more information on Precision , please visit:

http://?fltBrand[]=19&sort=20a

Ergonomically designed pipette for reverse pipetting - IKA pipettes

The grip is soft and comfortable
Feel comfortable: multiple ergonomic designs
Range lock: 4- digit display
Conical nozzle: durable shockproof

Solution for difficult sample pipetting: IKA pipettes are suitable for high density, high viscosity and volatile liquids. 1. Remove volatile samples: 1. Always rinse twice before pipetting; 2. Dispense as soon as possible after pipetting is completed. 2. Transfer high-viscosity sample: adopt the reverse pipetting mode: press the suction / discharge button to the second gear ( second stop point ) while aspirating , and press the button to the first gear when draining ( first stop point ) . In addition, a residence time of 3-5 seconds is required for both aspiration and drainage . 3. Pipetting high-density / low-density samples: The accuracy values ​​of pipettes are based on the transfer of pure water. If the density of the sample differs greatly from the density of water, the accuracy will be much worse. Therefore, it is necessary to first understand the density of the sample before pipetting, and then adjust the range to the product of the volume and density of the sample to be transferred. For example, if the density of a sample is 1.2g/cm3 and you need to transfer 300ul , then you should set the range to 360ul . This is only a rough adjustment method, and the strict adjustment method also needs to use the gauge or balance as an auxiliary tool for accurate calculation.

Sarms Tablets

We are one of the leading Sarms tablet suppliers in China with the best Sarms tablets for sale, specialized in providing high-quality products at low prices. We warmly welcome you to buy or wholesale bulk Sarms tablets for sale here from our factory. For a free sample, Our Sarms pills prices will surely meet your needs, please check out Sarms tablet main range products