The modern agriculture research of the 863 Program aims to improve the plant's ability to resist pests and diseases and kill harmful organisms. It has established a new research and development technology system for biological pesticides; the creation of genetic engineering bio-pesticides has significantly improved the effectiveness and production of biological pesticides. Research and construction of a platform for the creation of bio-pesticides with independent intellectual property rights, development of more than 20 new technologies and new processes, including new bio-pesticide active substance separation and identification technologies and safety assessment technologies, new biological pesticide fermentation processes and large-scale production processes, Insecticide and disease prevention-related functional gene cloning, expression and gene engineering biocontrol bacteria construction technology, new biological pesticide product formulations, compounding technology and new formulation stabilization technology, biological pesticide synergist development and industrialization technology. (1) Plant-derived pesticides: 15 new compounds with insecticidal bactericidal active ingredients were obtained from Sabina arborescens, and six species have been identified. Developed a new product of bio-pesticides or bio-chemical compounded pesticides such as sand and ground oil, aerosols, 0.15% podophyllotoxin microemulsion, 1.15% podophyllotoxin cyhalothrin microemulsion, and 0.75% podophyllotoxin EC. The plant membrane protective agent with antiviral function with the active ingredient of burdock leaf as the main component has been registered by the pesticide, and the new product has been popularized and applied for more than 100,000 mu. (2) Microbial Pesticides: The largest number of domestically-embracized fungal species was established in the country, and more than 2,200 strains were preserved. Through the preliminary screening of bioassays of important insect pests such as Monochamus alternatus, Anoplophora glabripennis, Dendrolimus punctatus, and peanut aphid, and further field control experiments, 20 strains of highly virulent strain producing strains for industrial fermentation were screened. (3) Agricultural antibiotics: A novel antibiotic, Qinlingmycin, was isolated and identified, and its producing bacteria is also a new species of Streptomyces. Antimycin A17 (5-1), an active ingredient that has a strong killing effect against Plutella xylostella and water spiders. The stable and high-yield strains of vallonomycin, tianshan sinensis and yammycin were obtained through mutagenesis techniques such as space and plasma injection. The liquid agent and granules of Men-myco-93-63 Streptomyces biocontrol agent with low pollution, low cost and good stability have been successfully developed. The prevention and control effect of the control effect was over 80%, which was significantly higher than that of the highly toxic pesticide aldicarb used in the current production, and the control effect against cucumber powdery mildew was more than 99%. (4) Induced pesticides: For the first time, the plant activator protein gene was cloned from the rice blast fungus, and the plant-activating protein Pichia transformation system was constructed to obtain genetically engineered strains, which provided a solid foundation for the industrialization of plant activator proteins. We studied the anti-pathogenic activity of 10 major volatile substances of tomato, and the production technology of burdock leaf chlorogenic acid. We have built a chlorogenic acid production line that processes 5 tons of burdock leaves every day, and an burdock leaf production plant that invests 10 million yuan Cangshan Zhuangwu Town is under construction. (5) Multifunctional genetic engineering biological pesticides: The chitinase gene and vip gene of Bacillus thuringiensis (Bt) were cloned, and an endophytic growth and disease prevention plant containing Bt VIP gene and chitinase gene was constructed. Health engineering bacteria. (6) A new technology for the large-scale production of fungal insecticides in industry was established. The new technology of gas phase dual-dynamic solid-state fermentation fundamentally solved the shortcomings of routine open fermentation, easy contamination of bacteria, difficult control of fermentation parameters, and unstable product quality; The process reduces the rate of contamination to below 0.1%, the fermentation level is 1-2 times higher than that of conventional solid state fermentation, and the production cost of the product is reduced by more than 50%. Silicone Surfactant,Liquid Silicone Adjuvant,Organo Silicone,Agro Spread Adjuvant Jiangxi Tiansheng New Materials Co.,Ltd , https://www.jxtsxcl.com