Technical parameters:
Technical parameters
MINGTAI LED760
MINGTAI LED560
Illuminance (lux)
180,000
130,000
Color Temperature (Kelvin)
4,300
4,300
Adjustable Color Temperature (Kelvin) (Optional)
3600-5600
3600-5600
Color Rendering Index (CRI)
96
96
Red Color Index (R9)
96
96
Light brightness control
standard
standard
Brightness control range
10-100%
10-100%
Temp rise (Surgeon head) (Celsius degree)
0.5
0.5
Temp rise (surgical field) (Celsius degree)
2
2
Effective Focusing Depth (cm)
60-150
60-150
Total Irradiance Ee (W/m2)
530
470
Radiant Power Ee/ Ec (mW/m2lux)
3.3
3.3
Lighting Spot Size (mm)
100-260
100-260
Bulb Type
LED
LED
Average Bulb Life (hours)
60,000
60,000
Bulb Quantity (main bulb+ auxiliary bulb)
128LEDS+384LEDS
56LEDS+168LEDS
Depth of illumination L1+L2 (cm)
130
130
Power Consumption (W)
70
50
Height adjustment (cm)
118
118
Power Input
100-240VAC, 50/60Hz
100-240VAC, 50/60Hz
Special advantage:
Protected by patent No. ZL 2013 2 0354946.9, do not infringe
1. High strength ABS material, ultra-thin light head, integrated surrounded 4 group handle design, meeting laminar flow requirements, easy to operate
2. Intelligent spot control technology: Light intensity not change when spot size changed
3. Intelligent shadow control technology: when light was sheltered by surgeon head, correlated LED light will automatically darken, other LED lights which are not sheltered will increase brightness, compensate the illuminance loss in surgery field, provide a better shadowless rate, bring the perfect surgical lighting (optional)
4. Variety of Surgical environment lighting mode: bright, normal, endoscopic lighting mode; Base axis has ambient light lighting mode (optional)
5. Satlite oval hanging main arm, meet laminar flow requirement
6. Double control system (optional), Li battery (optional), electric focus adjustment system (optional), RGBW color adjustment system (optional), independent R9 function (optional), manual focus adjustment function (standard)
7. Base configuration: Germany imported balance arm system, light head is designed by Germany engineer, USA LED bulb, Taiwan power system
Product feature:
1. Lamp shell is ABS material, integrated design with four handles around and streamlined ultra-thin design, the thickness is not more than 18CM, obtain excellent effect of laminar flow
2. Use international advanced warm white LED as shadowless illuminant, environmental, lower energy consumption LED bulb, bulb life: 60000 hours
3. LED do not engender infrared ray and ultraviolet radiation, it doesn't have the temperature rise and tissue damage caused by halogen shadowless light, can accelerate the wound healing after surgery, and has no Radiation pollution
4. LED color temperature is constant, soft and not dazzling, close to natural light
5. The main light use 128LEDS(main bulb)+384LEDS(spare bulb) independent LED illuminant (lamp cup is one main and three spare bulb structure), divided into multiple independent illuminant group, subsidiary light use 56LEDS(main bulb)+168LEDS(spare bulb) LED illuminant (lamp cup is one main and three spare bulb structure), divided into multiple independent illuminant group; every lamp use double CPU control, every group of LED illuminant controlled by special circuit chip, any group failure will not affect the normal use of shadowless light.
6. Double switch control system, when control panel failure, emergency switch which installed on light head can be used, guarantee light normal work. Control panel in the spring arm joint, film touch switch, easy to use, Not easy to damage
7. Microcomputer digital control, illumination can be adjustable; stepless adjustment, eight segments continuous indicating, adjustment range: 10%-100%, backlight illumination: 30 Lux; it offers illuminance memory function
8. Adopt restrain interference design of electromagnetic wave, avoid disturb with the other equipment in operating room
9. Use satlite oval swing pipe design, can install 4 cantilever devices in complex operating room, also can be reserved per hospital's request, convenient to upgrade and provide perfect solution for the increasing multi-media request of hospital
10. Balanced system using imported German Ondal arm module, more than 5 group universal joints, every cantilever must has more than 3 joints which can be rotated in 360 celsius degree, equipped with fatigue correcting unit and fix position hand handle device, easy to fix position after using for long time
11. The handle on the central of lamp can be detachable, can take high temperature (134 celsius degree) sterilization treatment, easily adjust, flexible fixed; Adopt balanced arm, handy operate, and make sure the lamp stable during operation; has C-arm joint control panel, has luminance prompt facility, provide environment lighting models
12. Lamp panel radius of gyration: 182cm, the lamp can be pulled to vertical floor, convenient to any angle illumination. Unify ABS operate handle and mid-operation handle of Lamp rim convenient to operate. Product execute international IEC standard.
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Human soluble CD23 molecule (sCD23) ELISA kit instruction manual
Human soluble CD23 molecule (sCD23) ELISA kit
 ( used in serum, plasma, cell culture supernatants and other biological fluids )
principle
This experiment used double antibody sandwich ABC-ELISA. The anti-human sCD23 mAb was coated on the microtiter plate, the sCD23 in the standard and the sample was combined with the mAb, and biotinylated anti-human sCD23 was added to form an immune complex attached to the plate, horseradish peroxidase The labeled Streptavidin is combined with biotin, the substrate working solution is blue, and finally the stop solution sulfuric acid is added. The OD value is measured at 450 nm, and the sCD23 concentration is directly proportional to the OD value. The concentration of sCD23 in the sample can be obtained by drawing a standard curve.
Kit composition ( 2-8 ° C preservation)
Coated Wells
96 holes
Enzyme Conjugate
12ml
10× specimen dilution (Sample Buffer)
12ml
20×Wash Buffer
50ml
Standards: 20ng/bottle
2 bottles
Substrate working fluid (TMB Solution)
12ml
Primary antibody working solution (Biotinylated Antibody)
12ml
Stop Solution
12ml
Prepare reagents and collect blood samples
1. Collect specimens: serum, plasma (EDTA), cell culture supernatant, tissue homogenate, etc., as soon as possible, store at 2-8 ° C for 48 hours; longer time must be frozen (-20 °C or -70 °C) Save to avoid repeated freezing and thawing.
2. Standard solution preparation: Add 1 ml of distilled water before use and mix well to prepare a 20 ng/ml solution. Set the standard tube 8 tube, the first tube plus the standard dilution 900ul, the second to the eighth tube to add the sample dilution 500ul. Add 100 ul of 20 ng/ml standard solution to the first tube, mix and aspirate 500 ul with the sampler, and transfer to the second tube. Repeat the dilution as described above, and remove 500 ul from the seventh tube and discard it. The eighth tube is a blank control.
3. The 10× specimen dilution was diluted 1:10 with distilled water (example: 1 ml concentrated dilution + 9 ml distilled water).
4. Washing solution: diluted 1:20 with distilled water (example: 1 ml concentrated washing solution added to 19 ml of distilled water)
Test procedure
1. Loading: Add 100 ul of standard or sample to be tested in each well. Mix the reaction plate thoroughly and let it stand at 37 °C for 120 minutes.
2. Wash the plate: Wash the plate thoroughly with washing solution 4-6 times, and dry it on the filter paper.
3. Add 100 ul of the first antibody working solution to each well. The reaction plate was thoroughly mixed and placed at 37 ° C for 60 minutes.
4. Wash the board: the same as before.
5. Add 100 ul of enzyme-labeled antibody working solution per well. The reaction plate was placed at 37 ° C for 30 minutes.
6. Wash the board: same as before.
7. Add 100 ul of substrate working solution per well, set 37 The reaction was carried out in the dark at °C for 15 minutes.
8. Add 100 ul of stop solution to each well and mix.
9. Measure the absorbance at 450 nm using a microplate reader within 30 minutes.
Result calculation and judgment
1. All OD values ​​should be subtracted from the blank value before calculation.
2. Take the standard products 2000, 1000, 500, 250, 125, 62.5, 31.2, 0 pg/ml as the abscissa and OD as the ordinate. Draw on the coordinate paper and draw the standard curve.
3. Find the corresponding sCD23 content on the graph based on the OD value of the sample.
Kit performance
1. Sensitivity: The minimum sCD23 detection concentration is less than 18pg/ml.
2. Specificity: Recombinant or natural human sCD23 can be detected simultaneously. Does not cross-react with other human cytokines.
3. Repeatability: The coefficient of variation in both the plate and the plate is less than 10%.
Precautions
1. It is recommended to make double holes for the above standard holes and samples to be tested. The standard curve should be made at the same time for each measurement.
2. The washing process is critical. Insufficient washing will result in an accuracy error and an erroneous rise in the OD value.
3. After the slats are opened, the remaining slats should be sealed again to keep the slats dry .
4. This kit should be stored in a 4 o C refrigerator.
5. This kit is for scientific research only and cannot be used for clinical diagnosis!