Phytase after processing

In recent years, phytase has been widely used in feed. In the production technology of phytase, the domestic production of phytase fermentation enzyme has reached the international advanced level, and some even far exceed foreign; as a post-processing technology, the domestic phytase producers have also made remarkable progress. At present, the main methods for post-processing of phytase in China include adsorption drying, spray drying, granulation, and coating methods. Adsorption drying is the earliest production method of phytase in China. At that time, the domestic phytase industry had just started and the fermentation enzyme activity remained at a relatively low level. It was the most economical production method to produce low-enzyme active products by the adsorption and drying method, and it was also the main production of the current low-activity enzyme products. the way. Adsorption drying is to mix the fermentation broth and corncob powder at a certain ratio and use two-step air drying. The first step uses hot air at 130°C for 10 seconds to dry; the second step uses air at 60°C to 80°C for 10 seconds to dry. This not only ensures the rapid dehydration of phytase, but also ensures a low loss of enzyme activity. Adsorption and drying of the product are greatly affected by the fermentation enzyme activity and the carrier. Quality control must be done to ensure the carrier's moisture, particle size, and color. Adsorption drying method of production of products, the cost is lower, but the phytase molecules are in direct contact with the outside world, the time stored in the feed is not as good as granulation and envelope type of phytase is generally suitable for the feed preservation time requirements are not high, Feed storage temperature and humidity environment better place. Spray drying is currently the main production method for domestic high enzyme products. For example, 2500 U/g, 5000 U/g product, the production method is to paste the fermentation broth and the carrier to form a certain proportion of the paste, under a certain pressure through the nozzle, dispersed into small droplets. These droplets are instantly dehydrated in a stream of hot air, forming a granular product. Because of the instantaneous dehydration, there is little loss of enzyme activity. At the same time, by adjusting the proportion of fermentation broth and carrier, different concentrations of phytase products can be produced. The products produced by spray drying should belong to the second generation in the country. It has the advantages of beautiful appearance, better fluidity and higher enzyme activity. When used in feed, it can produce more formula space. The disadvantage is that the particle size is not easy to control in the production process and there is a certain amount of dust during use. At the same time, similar to adsorption-dried products, phytase molecules are in direct contact with the outside world and are susceptible to other components in the feed. There are certain problems in the preservation. With the improvement of domestic phytase technology, domestic enterprises have successfully produced pellets and coated phytase. The advent of pellet and coated phytase indicates that the domestic phytase post-processing technology has reached the international advanced level. The pellet phytase is prepared by mixing the treated fermentation broth and the carrier in a certain proportion, and forming the product through drying at a low temperature and drying. Equivalent to the phytase molecules enclosed in a number of cells, isolation of the contact between the molecules and the outside world is more conducive to the preservation of phytase. Phytase is also more easily distributed in the feed due to the guaranteed particle size distribution and number of particles. There is no longer a problem with dust during use. The key to pellet phytase technology is the choice of carrier and drying method. The choice of carrier, first of all requires that the produced particles have a certain hardness, and the particles can rapidly disintegrate in water, releasing the phytase molecules, and play a role. Second, the chosen carrier must ensure stability in the feed. After the feed is mixed into the feed, it will not interact with the feed and affect the stability of the phytase. Finally, since the carrier has an impact on the activity of the enzyme, the determination of the vector requires a lot of calculations and experiments to get the correct result. The evaluation index of pellet phytase quality, including the number of particles per unit weight and particle size distribution. The number of particles per unit weight indicates the pellet's ability to distribute in the feed. If the number of particles is small, it is difficult to ensure uniform distribution after adding the feed. The greater the number of particles, the greater the chance of distribution in each part of the feed, in order to ensure the distribution of phytase in the feed in order to ensure the animal's feeding effect. The particle size distribution illustrates the uniformity of product size. The more concentrated the particle size, the more uniform the particle size of the product, when mixed into the feed, can better ensure the distribution of phytase in the feed. The coating uses the coating granules after forming the pellet phytase to form a firm and easily soluble film on the surface to isolate the phytase particles from the outside air and moisture. It is more conducive to the stability of phytase and the level of high temperature resistance is also improved to a certain extent. Coating agents are divided into two types of water-soluble and fat-soluble. It is best to use water-soluble coating agents to ensure rapid dissolution in the digestive tract and release of phytase. For example, fat-soluble coating agents, such as various types of fats or stearates, need to be effected by lipase in order to be released in large quantities; on the other hand, fats or stearates are easily oxidized in feed, affecting plant growth. The retention rate of acidase. The coating process must form a firm film on the surface, which is moisture-proof and insulates the air. This requires specific requirements for the coating time and process. For the evaluation of the quality of coated phytase products, the number of particles per unit weight and the particle size distribution are similar to those of granular phytase, and whether the coating is complete or not requires the use of microscopy.

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