CultreCoat® Extracellular Matrix Protein Array Cell Adhesion Assay Kit Principle

Cell adhesion is the related role between cells and extracellular machinery molecules. It plays an important role in tumor cell metastasis, infiltration, embryogenesis, etc. At the same time, many cells are needed in the process of normal cell growth and tissue differentiation. The adhesion function works. In the process of tissue gap transfer of tumor cells, the extracellular matrix is ​​a necessary part of it. Extracellular matrix ECM is an intricate network of macromolecules. Tumor cells need to interact with ECM before and during metastasis, and the cytoskeleton associated with cell mobility forms a complex, which ultimately performs metastasis. The extracellular matrix is ​​mainly composed of collagen (Collagen), non-collagen glycoprotein (such as fibronectin, lignin).

Conventional cell adhesion assays are currently performed by including extracellular matrix proteins (or individual components) embedded in cell culture plates. However, these traditional methods of cell behavior analysis have been criticized by researchers because of the following defects: 1. Higher experience on the operator, such as uniform deposition of matrix proteins and optimization of reagents and processes, it is difficult to achieve higher The stability of the experimental batch; 2, the cell matrix used for purchase did not undergo strict quality verification, and had great influence on cell behavior and even activity, and the final data lacked credibility; 3. Manual cell counts existed in certain random experiments. Sex. Here, Agilent Technologies recommends Trevigen's CultreCoat® and Cultre® full range of tumor cell behavioral assays to provide cell invasion, cell migration, cell adhesion, in vitro vascular regeneration, and vascular permeability analysis. And other complete tumor cell behavior analysis products and kits.

The cell adhesion analysis solution provided by Trevigen has the following characteristics:

1. Researchers can directly purchase pre-embedded kits of matrix components of different components of CultreCoat according to their own application and needs, or they can only purchase the Cultrex basement membrane extract components after strict biological function test;
2. Since different cell lines have multiple adhesion potentials for different matrix components, Trevigen also offers the CultreCoat Extracellular Matrix Protein Array Cell Adhesion Assay Kit designed to select the most appropriate BME component;
3. Trevigen's cell adhesion assay provides fully quantified cell adhesion analysis without the need for manual cell counting; Trevigen's patented BME matrix and other component proteins are pre-embedded on microplates with very low cytotoxicity. It is verified by strict bacteria/fungus/mycoplasma and is close to the real environment. At the same time, the fluorescence quantification scheme can ensure higher precision.

The CultreCoat® Extracellular Matrix Protein Array Cell Adhesion Assay Kit provides pre-embedded collagen IV, laminin I, BME components, collagen I, fibronectin, and vitronectin on a single microplate. As a matrix, the user obtains the adhesion of the cells to different extracellular matrix proteins through the matrix protein array analysis, thereby providing a reference for the subsequent in-depth analysis of selecting the most suitable matrix protein. The assay is simple and uses a standardized 96-well high-throughput assay. The black strip holes reduce the background and provide greater sensitivity and flexibility to the number of samples evaluated. The Calcein-AM label provides a direct comparison of cell load and adherent cell volume, based on the final fluorescence estimate to provide a load reference and percentage of adherent cells per well.

Amy Jet provides you with a one-stop cell adhesion analysis product and protocol, including cell adhesion assay kits for pre-embedded BME and various extracellular matrix proteins; in addition, for some of the most suitable substrates The initial experiment of the exoprotein also provides the CultreCoat Matrix Protein Array Cell Adhesion Assay Kit, which can be used for adhesion analysis of extracellular matrix proteins for screening, thus providing a solution for selecting the most suitable extracellular matrix protein. In addition, Amy Jet also offers Cultrex Base Membrane Extract (BME) and individual component protein products from Trevigen and Southern Biotech. If you are interested in the above products and solutions, please contact Ai Meijie Technology Co., Ltd. for laboratory solutions related to cell adhesion analysis, or request the latest product information.

Trevigen is a fast-growing US biotechnology company focused on oncology research products and services for cell-wide assays, apoptosis, DNA damage and repair, tumor cell function and behavior. As the general agent of Trevigen in China, Amy Jie and Trevigen provide Chinese researchers with the best and latest quality products and technical services in the fields of oxidative stress, cell damage and tumor cell behavior research.

Ai Meijie Technology Co., Ltd.

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